Thermo Fisher Scientific MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73)
Recombinant rabbit monoclonal antibody specific for MMP9, offering high specificity and sensitivity. Validated for WB, IHC, ICC/IF, and Flow Cytometry. Consistent lot-to-lot performance and animal origin-free formulation for reliable research use.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 5 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA80-73 |
| Immunogen | Synthetic peptide within Human MMP9 (aa 71–120) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809982 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed from immunoreactive rabbit DNA sequences. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
MMP9 (Matrix Metallopeptidase 9) is a zinc and calcium-dependent endopeptidase that degrades extracellular matrix proteins.
Key characteristics:
- Secreted as a 92 kDa zymogen; active form is 82 kDa
- Contains gelatin-binding, catalytic, collagen-homologous, and hemopexin-like domains
- Expressed by multiple cell types (monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, endothelial cells)
- Involved in inflammation, tissue remodeling, wound healing, tumor growth, and metastasis
- Associated with idiopathic atrial fibrillation and aortic aneurysm progression
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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