Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (HA58), eBioscience
인간 CD54(ICAM-1)를 인식하는 HA58 클론의 단일클론 항체로, Flow Cytometry, Western Blot, IHC 등에 사용 가능. 0.25 µg/test 권장. Affinity chromatography로 정제된 액상 항체이며, PBS(pH 7.2)에 0.09% sodium azide 포함. 4°C 보관.
- 카탈로그번호
- 14-0549-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (HA58), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 2 |
| Immunohistochemistry (IHC) | Assay-Dependent | 3 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 0.25 µg/test | 10 |
| ChIP assay (ChIP) | - | 1 |
| Neutralization (Neu) | Assay-Dependent | 1 |
| Functional Assay (Functional) | Assay-Dependent | 1 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HA58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467304 |
Available Conjugated Formats
- Alexa Fluor 700
- APC
- Biotin
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Yellow 610
- NovaFluor Yellow 690
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90–110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, and endothelial cells. CD54 expression increases on activated lymphocytes, and its interaction with ligand CD11a plays a key role in inflammatory responses.
Applications Reported:
Flow cytometry, Western blot, and immunohistochemistry. It has also been used to block in vitro mixed lymphocyte reactions (use Functional Grade HA58, Product #16-0549-82, for functional assays).
Applications Tested:
Tested in flow cytometry with normal human peripheral blood cells. Recommended ≤0.25 µg per test (final volume 100 µL). Optimal titration is recommended for specific assays.
Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein composed of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus. ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases during inflammation and is also observed in certain carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.