Thermo Fisher Scientific IFN gamma Monoclonal Antibody (ABM153)
사람 IFN gamma를 인식하는 마우스 단클론 항체로, IHC(P) 및 ELISA에 적합합니다. 단백질 A로 정제된 비결합 항체이며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| ELISA | 0.01–0.1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ABM153 |
| Immunogen | A synthetic peptide corresponding to the internal segment of human IFN gamma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: PBMC cells treated with cell stimulation cocktail
- Cellular location: Secreted
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemagglutinin, and other cytokines.
It is a dimeric glycoprotein composed of two 146 amino acid subunits, forming a functional homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as bands of 25, 20, and minor 15.5 kDa due to differential glycosylation.
The biological activity of IFN gamma is species-specific; human IFN gamma does not cross-react with mouse IFN gamma.
Functions include antiviral and tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhancement of immunoglobulin secretion by B lymphocytes.
Activation occurs through binding to IFN gamma receptor I and II, triggering the JAK-STAT pathway.
Human IFN gamma shares approximately 40% sequence homology with mouse IFN gamma and is upregulated by IL-2, FGF basic, and EGF, while downregulated by vitamin D3 or DMN. Gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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