Thermo Fisher Scientific CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), eBioscience

Applications

Western Blot (WB)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
• View 1 publication

Product Specifications

Additional Formats

• FITC
• PE
• Request custom conjugation

Product Specific Information

The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha).
CD181 is a 67–70 kDa member of the 7-transmembrane G-protein coupled receptor (GPCR) family.
It forms homodimers or heterodimers with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, subsets of T cells, mast cells, monocytes, endothelial cells, megakaryocytes, and oligodendrocytes.
Binding of ligands such as IL-8, NAP-2, GCP-2, and GRO-alpha induces cell activation, chemotaxis, proliferation, and angiogenesis.
CD181 and CD182 both mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospholipase D activation and respiratory burst.
IL-8 predominantly acts through CD181 to regulate neutrophil functions.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of normal human peripheral blood
• Recommended ≤ 0.5 µg per test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirical determination required)
• Antibody should be titrated for optimal performance

Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CXCR1 is a G-protein-coupled receptor for interleukin 8 (IL8).
It binds IL8 with high affinity and transduces signals via a G-protein activated second messenger system.
Mouse knockout studies suggest CXCR1 inhibits embryonic oligodendrocyte precursor migration in developing spinal cord.
IL8RB (another high-affinity IL8 receptor gene) and IL8RBP (pseudogene) form a gene cluster mapped to chromosome 2q33–q36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.