Thermo Fisher Scientific LAMP2 Monoclonal Antibody (GL2A7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1-20 µg/mL

-

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Antibody

Clone

GL2A7

Immunogen

Purified preparation of mouse liver lysosomal membranes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LAMP2 (CD107b),uniProtId:P17047-1,ncbiNodeId:10090,antigenRange:1-415,antigenLength:415,antigenImageFileName:MA1-165_LAMP2_CD107b_P17047-1_Rat.svg,antigenImageFileNamePDP:MA1-165_LAMP2_CD107b_P17047-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609332

Product Specific Information

MA1-165 detects LAMP2 in mouse samples.

MA1-165 has been successfully used in Western blot, immunofluorescence and flow cytometry applications.

The MA1-165 immunogen is a purified preparation of mouse liver lysosomal membranes.

Target Information

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon`s disease).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.