Thermo Fisher Scientific POLE3 Monoclonal Antibody (3C5.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mustelid, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3C5.1

Immunogen

Mono S FPLC column purified HeLa cell polymerase epsilon.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2166749

Product Specific Information

This antibody does not detect drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on s.cerivisae pol epsilon.

Target Information

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.