Thermo Fisher Scientific HIF1A Monoclonal Antibody (H1alpha 67-7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

H1alpha 67-7

Immunogen

Fusion protein containing residues 432-528 of human HIF-1 alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF1A,uniProtId:Q16665-1,ncbiNodeId:9606,antigenRange:432-528,antigenLength:826,antigenImageFileName:MA1-16517_HIF1A_Q16665-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16517_HIF1A_Q16665-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568571

Product Specific Information

In Western blot, multiple bands may be seen at 120 kDa representing post-translational modification of HIF-1 alpha.

Nuclear extracts are recommended.

Suggested positive control: Cos7 CoCl2-treated nuclear extract.

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.