Thermo Fisher Scientific CCR7 Monoclonal Antibody (4B12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µg/1X10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4B12

Immunogen

Rat basophilic leukaemia (RBL)-2H3 transfectants expressing mouse CCR7 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCR7,uniProtId:P47774-1,ncbiNodeId:10090,antigenRange:1-378,antigenLength:378,antigenImageFileName:MA1-163_CCR7_P47774-1_Rat.svg,antigenImageFileNamePDP:MA1-163_CCR7_P47774-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536868

Product Specific Information

MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes.

MA1-163 has been validated for Western blot on Jurkat cell lysates.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.