Thermo Fisher Scientific TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience
인간 배아줄기세포 및 암세포에서 발현되는 Podocalyxin 인식 항체. 다양한 응용(WB, IHC, ICC, Flow, IP)에 사용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 신뢰성 높은 결과 제공. 비접합 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 9 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 6 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TRA-1-81 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865303 |
Available Formats:
Product Specific Information
Description:
The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). The epitope is lost upon differentiation and is resistant to neuraminidase digestion, unlike TRA-1-60. It specifically binds a carbohydrate epitope on podocalyxin, a CD34-related sialomucin found on podocytes and associated with cancer aggressiveness (e.g., breast, prostate cancer).
Applications Reported:
Flow cytometry, immunoprecipitation, immunoblotting, immunofluorescence.
Applications Tested:
- Flow cytometry: ≤0.5 µg/test (2102Ep cell line)
- Immunoblotting: Band at 200–250 kDa (reducing/non-reducing)
- Immunofluorescence: ≤5 µg/mL (formaldehyde-fixed, permeabilized cells)
Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TRA-1-81 is a cell surface antigen expressed with SSEA-3, SSEA-4, and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). These markers are down-regulated during differentiation, whereas SSEA-1 appears after retinoic acid-induced differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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