Thermo Fisher Scientific Granzyme B Monoclonal Antibody (496B), eBioscience
인간 Granzyme B를 인식하는 단클론 항체로, 세포독성 T세포 및 NK세포 연구에 적합합니다. Western blot, IHC, ICC/IF 등에 사용 가능하며, 고순도(>90%)로 제조되었습니다. 액상 형태로 PBS buffer에 보존되어 연구용으로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Dog, Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 496B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865305 |
Available Conjugated Formats
- Alexa Fluor 420
- Alexa Fluor 514
- Alexa Fluor Plus 555
- Alexa Fluor Plus 647
- Request custom conjugation
Product Specific Information
The 496B antibody reacts with human Granzyme B (GrB), a serine protease located in the granules of cytotoxic T cells and NK cells. It is also known as CGL1 (cathepsin G-like-1) and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1). Granzyme B is essential for inducing apoptosis in target cells through interaction with cytotoxic T cells via the mannose 6-phosphate receptor, which functions as a death receptor.
Applications Reported:
This antibody is suitable for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections and for immunocytochemistry.
Applications Tested:
Validated for IHC on formalin-fixed paraffin-embedded human tissue with low pH antigen retrieval at ≤5 µg/mL. Optimal dilution should be determined by the user.
Purity: Greater than 90%, as determined by SDS-PAGE
Aggregation: Less than 10%, as determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Granzyme B is a member of the granzyme serine protease family found in cytotoxic T cells and NK cells. It induces apoptosis by activating caspase-3 and the mitochondrial pathway via Bid cleavage. Granzymes are neutral serine proteases stored in lytic granules of CTLs and NK cells. In humans, fewer granzymes have been cloned compared to mice.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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