Thermo Fisher Scientific c-Met Monoclonal Antibody (eBioclone 97), eBioscience

Thermo Fisher Scientific c-Met Monoclonal Antibody (eBioclone 97), eBioscience

Applications and Tested Dilution

• Western Blot (WB): -
• Flow Cytometry (Flow): 1 µg/test

Publications

• View 1 publication
• View 3 publications

Product Specifications

Additional Formats

• Biotin
• FITC
• Request custom conjugation

Product Specific Information

Description:
The eBioclone 97 monoclonal antibody was generated against a human c-Met-Ig fusion protein and reacts with human c-Met (HGFR)-transfected cells. Human c-Met is a 145 kDa receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver, and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation and activation of downstream effectors including ERK-1, ERK-2, and PLCγ. Activation of the c-Met pathway leads to cellular responses such as motility, scattering, proliferation, survival, and angiogenesis. Mutations in c-Met are implicated in various malignancies.

Applications Reported:

• Reported for use in flow cytometric analysis.

Applications Tested:

• Tested by flow cytometric analysis of human c-Met-transfected cells.
• Recommended usage ≤ 1 µg/test (100 µL final volume).
• Cell number: 10⁵–10⁸ cells/test (empirical determination recommended).
• Antibody should be titrated for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MET (cMET):
MET is a receptor-like tyrosine kinase associated with multiple human malignancies. Upon ligand activation, MET interacts with PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3, activating signaling cascades including RAS-ERK, PI3K-AKT, and PLCγ-PKC. MET is involved in embryonic development (gastrulation, muscle and neuron development, angiogenesis, kidney formation) and adult processes (wound healing, organ regeneration, tissue remodeling). Dysregulation of MET is linked to gastric, renal, and breast cancers, making it a key target in cancer therapeutics and diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.