Thermo Fisher Scientific Rat anti-Mouse IgD Secondary Antibody, FITC

Applications

Tested Dilution

Flow Cytometry (Flow)

0.1-0.2 µg per 1x10^6 cells in 100 µL

Product Specifications

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

11-26c

Immunogen

Gamma Immunoglobins Heavy chains

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

RRID

AB_2539780

Target

IgD

Antibody Form

Whole Antibody

Product Specific Information

This clone reacts with the delta heavy chain of mouse IgD of all haplotypes (e.g., IgDa , IgDb , IgDc ), and does not react with other immunoglobulin isotypes.This clone does not induce proliferation of mature B cells in vitro, nor does in vivo injection of the monoclonal antibody have any effect on activation of mature B lymphocytes.

Light exposure should be avoided for fluorochrome-conjugated reagents. Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.

The amount of antibody is determined by measuring the optical density using a spectrophotometer. The antibody titer is verified by immunofluorescent staining and flow cytometric analysis.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.