
Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Recombinant Rabbit Monoclonal Antibody (1H21L4)
Phospho-S6 (Ser235/236) 항체는 인간 RPS6 단백질의 인산화 상태를 검출하는 재조합 토끼 모노클로날 항체입니다. Western blot, ICC/IF, Flow cytometry에 적합하며 높은 특이성과 일관성을 제공합니다. 단백질 A로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1H21L4 |
| Immunogen | Phosphopeptide corresponding to amino acids 229–238 of human RPS6 [pS235/236] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532475 |
Product Specific Information
- Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Ribosomes catalyze protein synthesis and consist of small (40S) and large (60S) subunits composed of RNA species and distinct proteins. The RPS6 gene encodes a cytoplasmic ribosomal protein, a component of the 40S subunit, belonging to the S6E family. RPS6 is a major substrate for protein kinases, with phosphorylation at multiple C-terminal serine residues induced by growth factors and mitogens. Dephosphorylation occurs during growth arrest. This protein contributes to cell growth and proliferation by regulating translation of specific mRNA classes. Multiple processed pseudogenes of this gene are dispersed throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: 701363_S6_P62753-1_Rabbit.svg, 701363_S6_P62753-1_Rabbit_PDP.jpeg)
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