Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Alexa Fluor 488, eBioscience
Alexa Fluor 488으로 표지된 MIH5 클론의 항-마우스 PD-L1 단클론 항체. Flow cytometry 및 IHC에 적합하며, B7-H1/PD-1 상호작용을 차단하는 기능성 항체. 0.5 mg/mL 농도, 4°C 암소 보관.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 27 publications |
| Neutralization (Neu) | - | View 3 publications |
| Functional Assay | - | View 1 publication |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH5 |
| Conjugate | Alexa Fluor 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811871 |
Available Formats
- Unconjugated
- Alexa Fluor 660
- APC
- Biotin
- Brilliant UV 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 786
- Functional Grade
- NovaFluor Red 700 / 755
- NovaFluor Yellow 690 / 700
- PE / PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation
Product Specific Information
Description:
The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family with a predicted molecular weight of ~40 kDa. B7-H1 is expressed on leukocytes including T, B, NK, and DC cells, and serves as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 inhibits T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported:
Use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (100 µL final volume). Optimize antibody titration for best performance.
Fluorescence Properties:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, negatively regulating T-cell receptor signaling. PD-L1 ligation decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in murine and human cancers and upregulated by IFN-γ, suggesting a role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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