Thermo Fisher Scientific Trop2 (EGP-1) Monoclonal Antibody (MR54), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

10 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MR54

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10852561

Product Specific Information

Description: This MR54 monoclonal antibody reacts with human Trop-2, which is also known as Tumor-Associated Calcium Signal Transducer 2 (TACSTD2) and EGP-1. This type I transmembrane glycoprotein is expressed on trophoblast (syncytiotrophoblast and cytotrophoblast), epithelial, and most human carcinoma cells. Trop-2 plays a role in calcium-mediated signaling. Trop-2 has also been reported as an oncogene, with expression being correlated with tumorigenesis, metastasis, and anchorage-independent growth.

Applications Reported: This MR54 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P), and immunocytochemistry (ICC).

Applications Tested: This MR54 antibody has been tested by flow cytometry and immunocytochemistry on MCF7 cells. This can be used at less than of equal to 10 µg/mL. For immunohistochemistry of formalin-fixed paraffin embedded tissue, either low or high pH antigen retrieval can be used, although high pH antigen retrieval results in better staining intensity. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.