
Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), Alexa Fluor 488, eBioscience
마우스 Ly-6C 단백질을 인식하는 단클론 항체로, Alexa Fluor 488 형광 표지. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 0.125 µg/test 농도로 사용. 4°C 어둠 보관, 동결 금지. 연구용으로만 사용 가능.
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Applications
- Flow Cytometry (Flow): Tested at ≤ 0.125 µg/test
(A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number range: 10⁵–10⁸ cells/test.)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HK1.4 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574426 |
Additional Formats
- APC
- APC-eFluor 780
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Custom conjugation available
Product Specific Information
The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and a subset of lymphocytes. Expression can vary among mouse strains:
- Ly6C.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
- Ly6C.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells
In vitro addition of HK1.4 antibody can increase proliferation and stimulate cytokine release.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes at ≤ 0.125 µg/test
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Granulocytes are white blood cells characterized by cytoplasmic granules. Also known as polymorphonuclear leukocytes (PMN), they include neutrophils, which are the most abundant. Granulocytes are released from bone marrow under regulation by complement proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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