Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series
• NovaFluor Blue, Red, Violet, UV variants
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer.

• CD8αβ is found on most thymocytes and αβ TCR T cells.
• CD8αα is expressed on γδ TCR T cells, intestinal IELs, and dendritic cells.
  CD8 binds MHC class I and associates with p56lck, playing a role in T cell development and activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)

Recommendations:

• Titrate antibody for optimal staining.
• NovaFluor dyes are not compatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI).
  Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample (10³–10⁸ cells) or per antibody cocktail.

NovaFluor Technology:

• Based on Phiton™ nucleic acid dye structures.
• Engineered fluorescent signatures minimize spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8):

• Type I transmembrane glycoprotein in the immunoglobulin receptor family.
• Functions as a co-receptor with TCR for MHC class I molecules.
• Expressed on T cells, NK cells, monocytes, and dendritic cells.
• CD8α chain binds MHC-I and recruits Lck kinase for signal transduction.
• Activated CTLs contribute to pathogen and tumor clearance.
• Differentiation enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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