Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 585, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 585, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV/Violet/Yellow series
• eFluor series (450, 506, 615, 660)
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436~780)
• NovaFluor Blue/Red/UV/Violet/Yellow variants
• Custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, an approximately 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers.
CD8 binds to MHC class I and, through association with protein tyrosine kinase p56lck, plays a role in T cell development and activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.5 µg/test, 100 µL final volume)

Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Important Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01)

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample (regardless of number of NovaFluors)
• For single-color controls: add 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for minimal spillover and spread impacts.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family.
It functions as a co-receptor with TCR for MHC class I molecules and plays an essential role in T cell activation and differentiation.

• Predominantly expressed on T cells as CD8αβ heterodimers
• Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers
• CD8 ligation triggers Lck recruitment, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation
• Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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