Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 610-30S, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 610-30S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 1 publication

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV/Violet/Yellow series
• NovaFluor Blue/Red/Violet/UV series
• PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5
• Super Bright series
• Custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule (32–34 kDa), expressed as CD8αβ heterodimers or CD8αα homodimers.

• Major expression: thymocytes, mature αβ TCR T cells (CD8αβ), γδ TCR T cells, intestinal IELs, dendritic cells (CD8αα)
• CD8 binds MHC class I and associates with p56lck for T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Recommended Reagent:
Each NovaFluor conjugate ships with CellBlox Blocking Buffer.

• Add 5 µL per sample for staining or single-color controls.
• Add to antibody cocktails before combining with cells.

Optical Properties:
Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimized spillover.

Learn more about NovaFluor dyes

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein acting as a co-receptor for MHC class I molecules.

• Predominantly expressed on cytotoxic T cells (CD8αβ heterodimers).
• Also found on NK cells, monocytes, and dendritic cells (CD8αα homodimers).
• CD8-MHC-I interaction recruits Lck kinase, inducing lymphokine production and CTL activation.
• CTL differentiation enhanced by IL-2, IL-12, and TGF-β1.
• Plays a key role in immune response and pathogen/tumor clearance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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