Thermo Fisher Scientific PLCG2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: Raji, Raw264.7
• Predicted reactivity: Mouse (94%), Rat (95%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor-mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.
There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1, PLC delta2, and PLCe.

PLC delta exists as four different isoforms. PLC delta1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC delta1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and alpha1B-adrenoreceptor signaling.

Mouse PLC delta1 is highly expressed in brain, heart, lung, and testis. PLC delta is abnormally accumulated in autopsied brains with Alzheimer’s disease (AD), suggesting that it may play a role in the pathology of AD. PLC delta2 is markedly expressed in type II intestinal metaplasia and in adenocarcinoma. When PLC delta2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benign yet evolves toward neoplastic transformation. Thus, PLC delta2 expression may be a possible marker of gastric malignant transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.