Thermo Fisher Scientific Granzyme K Polyclonal Antibody
Rabbit polyclonal antibody specific to Granzyme K for detection in mouse and rat samples. Validated for Western blot and ELISA. Affinity purified and supplied in PBS with glycerol. Suitable for cytoplasmic granule protein studies. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 27–264 of human GZMK (NP_002095.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.06 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804366 |
Product Specific Information
Immunogen sequence:
IIGGKEVSPH SRPFMASIQY GGHHVCGGVL IDPQWVLTAA HCQYRFTKGQ SPTVVLGAHS LSKNEASKQT LEIKKFIPFS RVTSDPQSND IMLVKLQTAA KLNKHVKMLH IRSKTSLRSG TKCKVTGWGA TDPDSLRPSD TLREVTVTVL SRKLCNSQSY YNGDPFITKD MVCAGDAKGQ KDSCKGDSGG PLICKGVFHA IVSGGHECGV ATKPGIYTLL TKKYQTWIKS NLVPPHTN
Positive Samples: Mouse liver, Rat liver
Cellular Location: Cytoplasmic granule
Target Information
Granzyme K (GrK) is a trypsin-like serine protease localized in cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa protease located on chromosome 5q11–12 near the granzyme A gene. Granzyme K cleaves at basic residues (arginine and lysine) and induces cell death independently of caspase activation, producing single-stranded DNA nicks. It is primarily expressed in thymus, lung, spleen, and peripheral blood leukocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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