Thermo Fisher Scientific CCL4 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-CCL4 항체로, Human MIP-1β 검출에 적합합니다. Western Blot 및 Sandwich ELISA에 사용 가능하며, 고순도 항원으로부터 제조되었습니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공합니다.
- 카탈로그번호
- 500-P38BBT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CCL4 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MIP-1β (CCL4) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930504 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-1β (CCL4). The antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
To detect Human MIP-1β by sandwich ELISA (100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, together with PeproTech Polyclonal Anti-Human MIP-1β (500-P38B) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-1β.
Western Blot:
For detection of Human MIP-1β by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human MIP-1β is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P38BBT-1MG is provided as 2 × 500 µg.
Target Information
CCL4 (Macrophage Inflammatory Protein 1-beta, MIP-1β) belongs to the intercrine beta (chemokine CC) family. It is involved in chemotactic and proinflammatory responses and immune homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins and recruits various inflammatory cells to sites of inflammation.
CCL4 and the related protein CCL3 regulate the trafficking and activation of inflammatory cells during immune responses to pathogens. While both affect monocytes similarly, CCL4 selectively attracts CD4+ lymphocytes and CCL3 attracts CD8+ lymphocytes. Both are potent chemoattractants for B cells, eosinophils, and dendritic cells. The processed form of CCL4 can down-modulate CCR5 receptor expression, inhibiting CCR5-mediated HIV-1 entry into T cells. CCL4 binds with high affinity to CCR5 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.