Thermo Fisher Scientific CCL3 Polyclonal Antibody, PeproTech
Human MIP-1alpha(CCL3)에 특이적인 Rabbit Polyclonal 항체로, Western Blot, ELISA, Immunostaining 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 염증 반응 및 면역세포 이동 연구에 적합.
- 카탈로그번호
- 500-P38-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CCL3 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA (ELISA) | 0.5–2.0 µg/mL | 2 publications |
| In vitro Assay (IV) | – | 1 publication |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MIP-1alpha (CCL3) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930497 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-1alpha (CCL3) matrix.
Sandwich ELISA:
To detect Human MIP-1alpha (CCL3) by sandwich ELISA, use 0.5–2.0 µg/mL of this antibody (100 µL/well). In conjunction with PeproTech Biotinylated Anti-Human MIP-1alpha (CCL3) (500-P38Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-1alpha (CCL3).
Western Blot:
For Western Blot analysis, use at 0.1–0.2 µg/mL. Detection limit for Recombinant Human MIP-1alpha is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines involved in host defense against bacterial, viral, parasitic, and fungal infections. They regulate trafficking and activation of inflammatory cells such as macrophages, lymphocytes, and NK cells.
MIP-1alpha selectively attracts CD8+ lymphocytes, while MIP-1beta attracts CD4+ lymphocytes. Both also act as chemoattractants for B cells, eosinophils, and dendritic cells. Active on both human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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