Thermo Fisher Scientific DYKDDDDK Monoclonal Antibody (29E4.G7), DyLight 549
DYKDDDDK(FALG) 태그 단백질 검출용 마우스 단클론 항체로 DyLight 549 형광 표지. Western blot, ICC/IF, ELISA 등에 사용 가능. 높은 특이성과 민감도를 제공하며, 단백질 발현 및 정량 분석에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:10,000–1:25,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29E4.G7 |
| Immunogen | Synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling. |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- Agarose
- Biotin
- DyLight 405
- DyLight 488
- DyLight 649
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against the FLAG™ epitope tag and is useful for detecting FLAG-tagged proteins in various assays. It recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy-terminus of target proteins in transfected or transformed cells.
Target Information
The DYKDDDDK (FLAG) tag is a short peptide sequence commonly used as a protein tag for purification, detection, and localization. It consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The tag can be fused to the N- or C-terminus of a protein, enabling easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is highly hydrophilic, minimizing interference with protein folding and function. It is widely used in molecular biology and biochemistry for protein-protein interaction studies, expression analysis, and localization experiments.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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