Thermo Fisher Scientific Phospho-AKT (Thr308) Monoclonal Antibody (18F3.H11), DyLight 549
Phospho-AKT (Thr308) 특이 단클론 항체로, DyLight 549 형광 표지되어 Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. 인간, 마우스 등 다양한 종에 반응하며, AKT1 pT308 인산화 형태를 인식. 연구용으로 세포 생존 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunohistochemistry (IHC) | 20 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:4,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 18F3.H11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein |
| Conjugate | DyLight™ 549 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at T308.
A BLAST analysis suggests cross-reactivity with AKT pT308 from most vertebrate species based on 100% homology with the immunizing sequence.
Cross-reactivity with AKT2 and AKT3 is likely to occur.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival.
Activated AKT1 inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets in various cell types, including cancer cells.
Activation occurs via insulin, PI3K, IGF1, and other growth factors.
AKT1 promotes cell survival by inactivating forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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