Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), DyLight 649
Phospho-AKT (Ser473) 단백질 검출용 DyLight 649 결합 단클론 항체로, Western blot, ICC/IF, Flow Cytometry, ELISA 등에 사용 가능. 인간, 생쥐, 랫드, 영장류 시료 반응. 높은 특이성과 재현성을 제공하며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:4,000–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17F6.B11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid repeated freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at S473. BLAST analysis suggests cross-reactivity with AKT pS473 from human, mouse, rat, and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources, AKT2, and AKT3 has not been determined.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. AKT1 is activated by insulin, PI3K, IGF1, and other growth and survival factors. It promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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