Thermo Fisher Scientific TCR H-Y (male antigen) Monoclonal Antibody (T3.70), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

T3.70

Conjugate

PE PE PE

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466267

Product Specific Information

Description: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation.

Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.