
Thermo Fisher Scientific TCR H-Y (male antigen) Monoclonal Antibody (T3.70), PE, eBioscience
H-Y 항원을 인식하는 TCR을 검출하는 Mouse 모노클로날 항체로, Flow Cytometry에 적합합니다. PE 형광체로 결합되어 있으며, 0.125 µg/test 농도로 최적화되어 있습니다. T 세포 발달 및 MHC 관련 연구에 활용됩니다.
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Thermo Fisher Scientific TCR H-Y (male antigen) Monoclonal Antibody (T3.70), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T3.70 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466267 |
Additional Formats
Product Specific Information
The T3.70 monoclonal antibody reacts with the transgenic αβ TCR that recognizes the male antigen H-Y in the context of H-2D^b.
A large fraction of T cells in H-Y TCR transgenic mice express this receptor.
These mice are widely used to study T cell development and the role of thymic MHC in CD4⁺ and CD8⁺ T cell differentiation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on CD11.3 line derived from thymic tumor of H-Y TCR transgenic mice, expressing H-Y TCR.
Recommended usage ≤ 0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor.
The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic MHC in CD4⁺ and CD8⁺ T cell differentiation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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