Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE, eBioscience
마우스 ICOS(CD278)에 특이적인 단클론 항체로, PE 형광 표지된 Flow Cytometry용 제품입니다. 활성화된 T세포의 공동자극 분자 분석에 적합하며, 0.25 µg/test 권장 농도로 사용됩니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 36 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E.17G9 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466273 |
Available Formats
- Unconjugated
- APC
- Biotin
- FITC
- Functional Grade
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell-specific molecule and a third member of the CD28/CTLA-4 family. ICOS is a 47–57 kDa homodimer expressed on activated T cells, providing potent costimulatory activity for T cell activation and proliferation, and is required for humoral immune response. It binds to its ligand B7h/B7RP-1 on activated APCs, including B cells, and may play a protective role in inflammatory autoimmune diseases. ICOS is also implicated in Th2 cell development.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on Con A-stimulated mouse splenocytes; ≤0.25 µg per test recommended.
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test). Titrate for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICOS (CD278) encodes a protein in the CD28/CTLA-4 receptor family. It functions as a homodimer and plays a key role in cell-cell signaling, immune response regulation, and cell proliferation. Mutations or dysregulation of ICOS are associated with immunodeficiency disorders such as Common Variable Immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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