Thermo Fisher Scientific Cytokeratin 18 (KRT18) Recombinant Rabbit Monoclonal Antibody (KRT18, 2808R)
인간 KRT18 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 고순도 Protein A/G 정제, 액상 형태로 4°C 보관. 비진단 연구용.
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Thermo Fisher Scientific Cytokeratin 18 (KRT18) Recombinant Rabbit Monoclonal Antibody (KRT18, 2808R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KRT18, 2808R |
| Immunogen | Recombinant human full-length KRT18 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8.
It is expressed in gastrointestinal, respiratory, urogenital tract tissues, endocrine and exocrine tissues, and mesothelial cells.
Cytokeratin 18 is mainly found in non-squamous epithelia, adenocarcinomas, and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18.
Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified.
Cytokeratin subtype expression patterns aid in distinguishing epithelial malignancies and are useful in tumor differential diagnosis, cytopathology, and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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