
Thermo Fisher Scientific FBXO21 Monoclonal Antibody (OTI1E1), TrueMAB
FBXO21 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. 재구성 후 1 mg/mL 농도로 사용하며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E1 |
| Immunogen | Full length human recombinant protein of human FBXO21 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The FBXO21 gene encodes a member of the F-box protein family characterized by a 40 amino acid F-box motif.
F-box proteins are part of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex, which mediates phosphorylation-dependent ubiquitination.
They are classified into three groups:
- Fbws: Contain WD-40 domains
- Fbls: Contain leucine-rich repeats
- Fbxs: Contain diverse interaction modules or no recognizable motifs
FBXO21 belongs to the Fbxs class. Alternative splicing results in two transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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