Thermo Fisher Scientific A-Raf Recombinant Rabbit Monoclonal Antibody (4U9L7)
A-Raf 단백질을 인식하는 토끼 유래 재조합 단클론 항체로 Western blot, IHC, ELISA에 적합. 인간 시료 반응성, HEK293 발현 시스템 사용. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4U9L7 |
| Immunogen | Recombinant fusion protein containing amino acids 166–290 of human A-RAF (P10398) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2912032 |
Product Specific Information
- Positive test controls include: HeLa, MCF7.
- Immunogen sequence:
RQHEAPSNRP LNELLTPQGP SPRTQHCDPE HFPFPAPANA PLQRIRSTST PNVHMVSTTA PMDSNLIQLT GQSFSTDAAG SRGGSDGTPR GSPSPASVSS GRKSPHSKSP AEQRERKSLA DDKKK
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is the cellular homolog of v-Raf, the transforming gene product of murine sarcoma virus strain 3611.
A-Raf, a second member of the Raf gene family, shows high homology to Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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