
Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody
Pyruvate Carboxylase를 인식하는 Rabbit Polyclonal Antibody로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성이 있으며, 액상 형태로 Affinity Chromatography로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 879–1178 of human Pyruvate Carboxylase (NP_001035806.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.96 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914556 |
Product Specific Information
- Positive test controls: HepG2, HeLa, MCF7, A-549, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain
- Subcellular location: Mitochondrion matrix
Immunogen sequence:
HSMGLGSKFK EVKKAYVEAN QMLGDLIKVT PSSKIVGDLA QFMVQNGLSR AEAEAQAEEL SFPRSVVEFL QGYIGVPHGG FPEPFRSKVL KDLPRVEGRP GASLPPLDLQ ALEKELVDRH GEEVTPEDVL SAAMYPDVFA HFKDFTATFG PLDSLNTRLF LQGPKIAEEF EVELERGKTL HIKALAVSDL NRAGQRQVFF ELNGQLRSIL VKDTQAMKEM HFHPKALKDV KGQIGAPMPG KVIDIKVVAG AKVAKGQPLC VLSAMKMETV VTSPMEGTVR KVHVTKDMTL EGDDLILEIE
Target Information
Pyruvate Carboxylase는 biotin과 ATP를 필요로 하며, pyruvate를 oxaloacetate로 카복실화하는 반응을 촉매합니다. 활성 효소는 미토콘드리아 매트릭스에 위치한 homotetramer 구조를 가지며, gluconeogenesis, lipogenesis, 인슐린 분비 및 신경전달물질 glutamate 합성에 관여합니다.
이 유전자의 변이는 Pyruvate Carboxylase 결핍과 관련이 있습니다.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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