Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:10-1:100

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_0010358061) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pyruvate Carboxylase,uniProtId:P11498-1,ncbiNodeId:9606,antigenRange:879-1178,antigenLength:1178,antigenImageFileName:PA5-120984_Pyruvate_Carboxylase_P11498-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120984_Pyruvate_Carboxylase_P11498-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.96 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914556

Product Specific Information

Positive test controls include: HepG2, HeLa, MCF7, A-549, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain. The target is usually found in the following locations: Mitochondrion matrix.

Immunogen sequence: HSMGLGSKFK EVKKAYVEAN QMLGDLIKVT PSSKIVGDLA QFMVQNGLSR AEAEAQAEEL SFPRSVVEFL QGYIGVPHGG FPEPFRSKVL KDLPRVEGRP GASLPPLDLQ ALEKELVDRH GEEVTPEDVL SAAMYPDVFA HFKDFTATFG PLDSLNTRLF LQGPKIAEEF EVELERGKTL HIKALAVSDL NRAGQRQVFF ELNGQLRSIL VKDTQAMKEM HFHPKALKDV KGQIGAPMPG KVIDIKVVAG AKVAKGQPLC VLSAMKMETV VTSPMEGTVR KVHVTKDMTL EGDDLILEIE

Target Information

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.