Thermo Fisher Scientific SOAT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SOAT1,uniProtId:P35610-1,ncbiNodeId:9606,antigenRange:1-130,antigenLength:550,antigenImageFileName:PA5-120985_SOAT1_P35610-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120985_SOAT1_P35610-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.03 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914557

Product Specific Information

Positive test controls include: H460, HeLa, SW480, Mouse stomach, Mouse liver, Rat liver, Rat stomach. The target is usually found in the following locations: Endoplasmic reticulum membrane, Multi-pass membrane protein.

Immunogen sequence: MVGEEKMSLR NRLSKSRENP EEDEDQRNPA KESLETPSNG RIDIKQLIAK KIKLTAEAEE LKPFFMKEVG SHFDDFVTNL IEKSASLDNG GCALTTFSVL EGEKNNHRAK DLRAPPEQGK IFIARRSLLD

Target Information

SOAT1 (sterol O-acyltransferase-1), also designated ACAT1, is a homotetra-meric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A (acyl-CoA). The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase-2), also known as ACAT2 (acyl-CoA:cholesterol acyltransferase-2), participates in lipo-protein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.