Thermo Fisher Scientific STAT5a Recombinant Rabbit Monoclonal Antibody (BLR124H)
STAT5a 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry, IP 등 다양한 응용 가능. 인간 및 마우스 반응성, HEK293F 발현 시스템에서 생산된 고순도 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC(P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 2 µL/10^6 cells |
| Immunoprecipitation (IP) | 20 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR124H |
| Immunogen | Peptide representing a region between residue 744 and the C-terminus (residue 794) of Human STAT5a |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus and act as transcription activators.
STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones.
Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and essential for tumorigenesis.
In mice, STAT5 alpha induces expression of BCL2L1/BCL-X(L), suggesting an antiapoptotic role. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, with differential mRNA accumulation noted in muscle and mammary tissues.
STAT5 alpha is critically involved in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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