Thermo Fisher Scientific Human IL-12 p35 Recombinant Protein
정제된 재조합 인간 IL-12 p35 단백질로 N-말단 His-tag 포함. 다양한 면역학적 분석에서 양성 대조군으로 사용 가능. E. coli 발현 시스템에서 생산되며 95% 이상 순도 보유. -20°C에서 보관하며 동결/해동 반복을 피해야 함.
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Applications
- Western Blot (WB): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Amino acids Arg23–Ser219 containing an N-terminal His-tag |
| Tag | His-tag |
| Class | Recombinant |
| Type | Protein |
| Purity | >95%, by SDS-PAGE under reducing conditions |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.19 mg/mL |
| Purification | Purified |
| Storage Buffer | 20 mM Tris HCl, pH 8, with 50% glycerol, 1 mM EDTA |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
This product contains purified recombinant human IL-12 p35 protein with an N-terminal hexahistidine tag.
It may be used as a positive control in various immunological assays.
Target Information
Interleukin 12A (IL-12A) is a signaling cytokine belonging to the Interleukin-6 protein superfamily.
The IL-12 alpha chain precursor is a 35-kDa subunit of the disulfide-linked heterodimeric cytokine IL-12 and exhibits diverse biological activities.
IL-12A can act as a growth factor for activated T and NK cells, enhancing the lytic activity of NK and lymphokine-activated killer cells.
It stimulates IFN-γ production by resting PBMCs and supports T-cell-independent induction of IFN-γ, aiding in the differentiation of Th1 and Th2 cells.
Additionally, IL-12A is involved in the positive regulation of tyrosine phosphorylation of Stat4 protein and plays a role in innate immunity signaling requiring nitric oxide synthase 2A.
Together with EBI3, IL-12A forms the heterodimeric cytokine interleukin-35 (IL-35), produced by T regulatory cells and essential for maximal suppressive activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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