Thermo Fisher Scientific p21WAF1 (Tumor Suppressor Protein) Monoclonal Antibody (CIP1/823)
p21WAF1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간 시료 반응성, 마우스 및 랫 반응 없음. p53 경로 연구 및 세포주기 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CIP1/823 |
| Immunogen | Recombinant full-length human CDKN1A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
p21 plays a crucial role in the cellular response to genotoxic stress and is a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits Cdk1 and Cdk2, halting the transition from G1 to S phase or G2 to mitosis, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic effects by binding to and inhibiting caspase 3, ASK1, and JNK.
p21 functions dually in carcinogenesis—acting as both a tumor suppressor and, in some contexts, an oncogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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