
Thermo Fisher Scientific Phospho-p21 (Thr145) Polyclonal Antibody
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p21 단백질의 Thr145 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 적용 가능. 고순도(>95%) 및 1 mg/mL 농도. 세포 내 DNA 손상 반응 및 CDK 억제 연구에 적합.
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Thermo Fisher Scientific Phospho-p21 (Thr145) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human p21 around the phosphorylation site of Threonine 145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553658 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 21 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
- Plays a key role in cellular response to genotoxic stress and is a major transcriptional target of p53.
- Nuclear p21 binds and inhibits Cdk1 and Cdk2, blocking cell cycle progression from G1 to S phase or G2 to mitosis after DNA damage, enabling DNA repair.
- Cytoplasmic p21 exhibits anti-apoptotic effects by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK.
- Functions as both tumor suppressor and oncogene, depending on cellular context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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