Thermo Fisher Scientific Phospho-c-Myc (Thr358) Polyclonal Antibody
c-Myc 단백질의 Thr358 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), IP에 적합합니다. Human, Mouse, Rat 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 증식 및 암 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Myc around the phosphorylation site of Threonine 358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553654 |
Product Specific Information
- This antibody detects endogenous protein at molecular weights of 49, 57, and 65 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and is involved in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK plays a key role in regulating cell proliferation and cycle control.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. Deregulated expression of c-Myc induces apoptosis and contributes to oncogenesis in multiple tissues, including lung, breast, colon, and neuroblastoma.
Translocation of the c-Myc locus is associated with Burkitt’s lymphoma and other B-cell proliferative disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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