Thermo Fisher Scientific UGT2B10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2720975

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

DNA containing double-strand breaks (DSB) created by the CRISPR/Cas9 system can be repaired by either the non-homologous end-joining (NHEJ) or the homology-directed repair (HDR) pathway. The NHEJ repair pathway introduces non-specific insertions or deletions at the cleavage site, whereas the HDR pathway allows for precise gene editing at the DSB site. Target-specific HDR Plasmids provide a DNA repair template for a DSB and, when co-transfected with CRISPR/Cas9 KO Plasmids, enable the insertion of specific selection markers where Cas9-induced DNA cleavage has occurred. The HDR plasmid can incorporate a Red Fluorescent Protein (RFP) gene to visually confirm transfection and an antibiotic resistance gene (puromycin) for selection of cells containing a successful CRISPR/Cas9 doublestrand break. The puromycin resistance and RFP encoding genes are flanked by two LoxP sites that are recognized by the Cre Vector, which can be used to later remove these selection markers from the genomic DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.