Thermo Fisher Scientific UGT2B10 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

DNA containing double-strand breaks (DSB) created by the CRISPR/Cas9 system can be repaired by either the non-homologous end-joining (NHEJ) or the homology-directed repair (HDR) pathway.
The NHEJ pathway introduces non-specific insertions or deletions at the cleavage site, whereas the HDR pathway enables precise gene editing at the DSB site.
Target-specific HDR plasmids provide a DNA repair template and, when co-transfected with CRISPR/Cas9 KO plasmids, allow insertion of selection markers at Cas9 cleavage sites.
These plasmids can include a Red Fluorescent Protein (RFP) gene for visual confirmation and a puromycin resistance gene for selection. Both genes are flanked by LoxP sites, which can be excised using the Cre vector.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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