Thermo Fisher Scientific BTK Monoclonal Antibody (OTI15G8), TrueMAB
BTK 단백질을 표적하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. Lyophilized 형태로 제공되며, 1 mg/mL 농도 및 PBS buffer 기반입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI15G8 |
| Immunogen | Full length human recombinant protein of human BTK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily.
Mutations in the BTK gene are associated with developmental blocks in human B-cell ontogeny and immunodeficiency disorders.
BTK interacts with toll-like receptor (TLR) family members such as TLR4, TLR6, TLR8, and TLR9, which are essential for innate and adaptive immunity.
It also interacts with key proteins involved in TLR4 signal transduction, including MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting a role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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