Thermo Fisher Scientific BTK Monoclonal Antibody (OTI10E10), TrueMAB
BTK 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. PBS buffer에 보관하며, 재구성 후 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10E10 |
| Immunogen | Full length human recombinant protein of human BTK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase in the SRC-related TEC subfamily. Mutations in the BTK gene are associated with severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders.
BTK interacts with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9, which are key molecules in innate and adaptive immunity. It also associates with proteins involved in TLR4 signaling (MyD88, TIRAP, IRAK) but not TRAF-6, indicating its role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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