Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 755, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2) differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and play a key role in presenting intracellular peptides to cytotoxic T lymphocytes.

HLA-A3 is expressed by 15–25% of cancer patients and has been shown to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Updated formulation for optimal NovaFluor staining.
• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for spillover and spread control.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

Target Information

HLA-A is a class I heavy chain molecule forming a heterodimer with beta-2 microglobulin.
It presents peptides derived from the endoplasmic reticulum lumen and is expressed in nearly all cells.

• Heavy chain ≈ 45 kDa
• Gene contains 8 exons
  • Exon 1: leader peptide
  • Exons 2–3: α1, α2 domains (peptide binding)
  • Exon 4: α3 domain
  • Exon 5: transmembrane region
  • Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide-binding specificity.
Typing for these polymorphisms is routinely performed in bone marrow and kidney transplantation.
Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without authorization.

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