Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 730, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Available Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 has two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes.

HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested for flow cytometry using normal human peripheral blood cells. Use 5 µL (0.6 µg) per test in a final volume of 100 µL. Recommended cell count: 10⁵–10⁸ cells/test.

Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

This product ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance. Add 5 µL per sample to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology, employing novel nucleic acid dye structures engineered for optimized fluorescence with minimized spectral spillover and spread.
Excitation: 552 nm; Emission: 718 nm; Laser: 561 nm (Yellow)

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. It forms a heterodimer with beta-2 microglobulin and is expressed on nearly all nucleated cells. The heavy chain (~45 kDa) has 8 exons encoding leader peptide, alpha domains, transmembrane region, and cytoplasmic tail. Polymorphisms in exons 2 and 3 determine peptide-binding specificity, crucial in transplantation compatibility and immunopeptidomics.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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