Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2) differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes. HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen hTERT.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for use with normal human peripheral blood cells at 5 µL (0.6 µg)/test.
A test is defined as staining a cell sample (10⁵–10⁸ cells/test) in a final volume of 100 µL.

Important Usage Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures for engineered fluorescent signatures minimizing spillover and spread.

Optical Properties:

• Excitation: 552 nm
• Emission: 663 nm
• Laser: 561 nm (Yellow)

Target Information

HLA-A is a class I heavy chain paralogue forming a heterodimer with beta-2 microglobulin.
It plays a central role in antigen presentation, expressed in nearly all cell types.
The heavy chain (~45 kDa) contains 8 exons encoding leader, alpha domains, transmembrane, and cytoplasmic regions.
Polymorphisms in exons 2 and 3 determine peptide binding specificity, relevant for transplantation typing and immunopeptidomics.
HLA and MHC antibodies are crucial for neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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