Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Yellow 730, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

Description:
The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 has two subtypes (A3.1 and A3.2) differing at amino acid positions 152 and 156.
MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes. HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells.
Recommended usage: 5 µL (0.6 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 552 nm; Emission: 718 nm; Laser: 561 nm (Yellow Laser).

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer of a heavy chain and beta-2 microglobulin.

• The heavy chain (~45 kDa) is membrane-anchored.
• Plays a central role in immune response by presenting peptides derived from the ER lumen.
• Expressed in nearly all cells.
• Gene contains 8 exons:
  • Exon 1: leader peptide
  • Exons 2–3: α1, α2 domains (peptide binding)
  • Exon 4: α3 domain
  • Exon 5: transmembrane region
  • Exons 6–7: cytoplasmic tail
• Polymorphisms in exons 2–3 determine peptide-binding specificity.
• HLA typing is routinely performed for bone marrow and kidney transplantation.
• HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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