Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion, expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Optimal titration is recommended.

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is intended for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Handling Notes:

• Light sensitive: Protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation: 350 nm; Emission: 660 nm; Laser: Ultraviolet Laser.

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD16 (FcγRIII) is a 50–65 kDa cell surface molecule expressed in two forms: a transmembrane form on NK cells and some T cells, and a GPI-linked form on granulocytes. It mediates antibody-dependent cellular cytotoxicity (ADCC).
CD32 (FcγRII) is a 40 kDa glycoprotein mediating endocytosis, secretion, cytotoxicity, and immunomodulation. Expressed by B cells, monocytes, granulocytes, and platelets. Variations in CD32 gene expression may be linked to susceptibility to systemic lupus erythematosus (SLE).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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