Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which forms a heterodimer with CD18. CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It plays a role in leukocyte adhesive interactions via binding to CD54, iC3b, and fibrinogen.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes (≤ 0.5 µg/test)
  • A test is defined as staining 10⁵–10⁸ cells in 100 µL
  • Optimize antibody titration for best performance

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye emitting at 660 nm, suitable for UV laser (355 nm) excitation. Ensure instrument compatibility.

Staining Panel Recommendation:
When using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11, along with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, granulocytes, and some lymphocytes

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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