Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, 5.5, 7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except for plasma cells. CD19 forms a complex with CD21, CD81, and MHC class II, associating with the BCR to mediate signaling that induces phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Fluorochrome: Brilliant Ultra Violet™ 661 (BUV661), emission at 660 nm, for UV (355 nm) laser-equipped cytometers.
• Handling: Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when combining polymer dyes to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal effect on signal.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on nearly all peripheral B cells. It can also appear on certain myeloid leukemia cells. CD19 acts as a co-receptor with the B cell receptor (BCR), lowering the activation threshold and amplifying antigen receptor signaling. Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity. It serves as a critical regulator of B cell proliferation and differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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