Thermo Fisher Scientific Sendai virus HN Monoclonal Antibody (1A6), Alexa Fluor 647, eBioscience
Sendai 바이러스 HN 단백질을 인식하는 Mouse 단클론 항체로 Alexa Fluor 647로 표지됨. Flow cytometry 및 ICC/IF에 적합하며, iPSC 재프로그래밍 과정 중 잔류 Sendai 바이러스 검출에 유용. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 647, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A6 |
| Immunogen | Sendai Virus |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848407 |
Available Formats
Product Specific Information
This 1A6 monoclonal antibody recognizes the hemagglutinin-neuraminidase (HN) protein of Sendai virus. It has been validated for use in flow cytometry and immunocytochemistry and has been reported to neutralize Sendai virus.
The CTS CytoTune-iPS 2.1 and 2.0 Sendai Reprogramming Kits use Sendai virus particles to deliver Yamanaka factors into target cells for reprogramming into induced pluripotent stem cells (iPSC). Clone 1A6 can be used to detect residual Sendai virus following transduction and culture.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of Sendai virus-infected cells. Recommended ≤0.06 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration empirically.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Target Information
Sendai virus is a single-stranded RNA respirovirus from the Paramyxoviridae family. It replicates in the respiratory tract and infects mammals such as mice, hamsters, rats, and guinea pigs. The Hemagglutinin-Neuraminidase (HN) protein mediates viral attachment and entry by binding and cleaving terminal sialic acids on host cell glycoproteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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