Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), Alexa Fluor 647, eBioscience
인간 및 생쥐 ZAP-70 단백질을 인식하는 Alexa Fluor 647 결합 단일클론 항체로, T세포 및 NK세포 신호전달 연구에 적합. 세포내 Flow Cytometry 및 ICC/IF에 사용 가능. 0.2 mg/mL 농도의 액상 형태로 제공되며, 4℃ 암소 보관.
- 카탈로그번호
- 51-6695-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), Alexa Fluor 647, eBioscience
Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), Alexa Fluor 647, eBioscience
Applications
- Immunocytochemistry (ICC/IF)
- Tested Dilution: Not specified
- View 1 publication
- Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- View references
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 647, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E7.2 |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784736 |
Additional Formats
Product Specific Information
The 1E7.2 antibody reacts with human and mouse ZAP-70, a cytosolic protein tyrosine kinase (PTK) in the Syk family. It is expressed in T and NK cells and is essential for TCR signaling and development. ZAP-70 binds to phosphorylated ITAMs on TCR complex subunits and becomes phosphorylated by autophosphorylation and by Lck kinase. Phosphorylation enhances kinase activity and downstream signaling. Mutations in ZAP-70 can lead to Severe Combined Immunodeficiency Syndrome (SCID).
The 1E7.2 clone was generated against a KLH-peptide corresponding to residues 282–307 of human ZAP-70. ZAP-70 expression correlates with mutated IgVH in B-CLL, serving as a prognostic marker measurable by intracellular flow cytometry.
Applications Reported:
- Intracellular flow cytometric analysis.
Applications Tested:
- Tested on human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
- Recommended ≤ 0.5 µg/test (100 µL final volume).
- Cell number: 10⁵–10⁸ cells/test (empirical determination required).
- Titrate for optimal performance.
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Target Information
ZAP-70 is a cytosolic tyrosine kinase of the Syk family, expressed in T-cells and NK cells. It is required for TCR activation. Upon TCR engagement, ZAP-70 is phosphorylated at tyrosines 292, 315, and 319.
- Tyrosine 315 (Vav binding site) and 319 (Lck binding site) enhance signaling.
- Tyrosine 292 attenuates activation and signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.