Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), Alexa Fluor 647, eBioscience

Thermo Fisher Scientific ZAP-70 Monoclonal Antibody (1E7.2), Alexa Fluor 647, eBioscience

Applications

• Immunocytochemistry (ICC/IF)
  • Tested Dilution: Not specified
  • View 1 publication
• Flow Cytometry (Flow)
  • Tested Dilution: 0.5 µg/test
  • View references

Product Specifications

Additional Formats

• Unconjugated
• FITC
• PE
• Request custom conjugation

Product Specific Information

The 1E7.2 antibody reacts with human and mouse ZAP-70, a cytosolic protein tyrosine kinase (PTK) in the Syk family. It is expressed in T and NK cells and is essential for TCR signaling and development. ZAP-70 binds to phosphorylated ITAMs on TCR complex subunits and becomes phosphorylated by autophosphorylation and by Lck kinase. Phosphorylation enhances kinase activity and downstream signaling. Mutations in ZAP-70 can lead to Severe Combined Immunodeficiency Syndrome (SCID).
The 1E7.2 clone was generated against a KLH-peptide corresponding to residues 282–307 of human ZAP-70. ZAP-70 expression correlates with mutated IgVH in B-CLL, serving as a prognostic marker measurable by intracellular flow cytometry.

Applications Reported:

• Intracellular flow cytometric analysis.

Applications Tested:

• Tested on human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product #00-5523-00).
• Recommended ≤ 0.5 µg/test (100 µL final volume).
• Cell number: 10⁵–10⁸ cells/test (empirical determination required).
• Titrate for optimal performance.

Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser

Target Information

ZAP-70 is a cytosolic tyrosine kinase of the Syk family, expressed in T-cells and NK cells. It is required for TCR activation. Upon TCR engagement, ZAP-70 is phosphorylated at tyrosines 292, 315, and 319.

• Tyrosine 315 (Vav binding site) and 319 (Lck binding site) enhance signaling.
• Tyrosine 292 attenuates activation and signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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